Data were expressed as mean percentage of viable cells. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. 314 low lamp level warning. 305 reference level saturation. Or scan up to 384 wells at any time to determine the best assay conditions. Fibrin clots were formed as described above with some modifications 46. 00 USD. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Values were determined according to a standard curve (log/log curve-fit). Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. The possi-ble effect of zinc or copper on the binding of heme by A was tested as described above by using a 50 and 100 M final concentration of each metal. Water – distilled water or equivalent. Labsystems Luminoskan RT Microplate Reader. application or budget. Near bottom. 5% (w/v) sodium dodecyl sulfate-polyacrylamide gels, and transferred to immobilon polyvinylidenedifluoride membranes (Millipore, Bedford, USA). Assay buffer was 50 m M Hepes, pH 7. The cells were pretreated, serum starved for 24 h, and then treated with NK-cdM priora SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). Molecular Devices SpectraMAX 340 Microplate Reader. . These models can no longer be repaired. 1 mL of acetylacetone. Analysis of ALA by Ehrlich’s Reagent Assay. 303 unable to cal dark current. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. The. Labsystems Luminoskan RT Microplate Reader. Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The ODs of wells loaded with DMEM plus 10% CS were subtracted as background. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. A. 5 ng/ml; estradiol: 10 pg/ml. All ELISA plates were analyzed using a SpectraMax 340 PC microplate reader (Molecular Devices). Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. Also for: Spectramax 190, Spectramax 340pc384, Versamax. The plate was covered and incubated for one hour at room temperature. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. Donor: 616/10 nm. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. Software (SoftMax Pro ® software or comparable plate reader software). Molecular Devices SpectraMax 340 PC 384 Microplate Reader. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)Bob - 06 Apr. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode Opening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. . Number of flashes. FlexStation® 3 Multi-Mode Microplate Reader. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. 5. About The SpectraMax GEMINI EM is controlled by an external computer running SoftMax ® Pro software which provides integrated instrument control, data display, and statistical data analysis. Statistical analysis. Description Control Panel Cuvette Chamber Back Panel Microplate Drawer The Control Panel TEMP on/off TEMP Figure 1. SoftMax Pro. #N207) LAL Reagent Water (Lonza cat. The reaction mixture was incubated for 5 min. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). 1 All Necessary Cables and Cords Manuals General Specifications Dimensions (in. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. SpectraMax Injector Cartridge with SmartInject™ Technology. ZERO BIAS - scores, article reviews, protocol conditions and moreAbsorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. used. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. Features showcased by this model include. Rate of fibrinolysis. Operators can conveniently select up to 6 wavelengths at a time for absorbance detection in the visible wavelength range 340 nm - 850 nm. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. 0 2. reader (Molecular Devi ces). Molecular Devices SpectraMax Gemini XS Microplate Reader. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andSPECTRAmax ® PLUS 384 Microplate Spectrophotometer. Manufacturer: Molecular Devices Model: SpectraMax The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. 97), and each plate contained at least six positive and six negative controls. The chamber contains. EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen. Spectramax 340pc User Manual, Hudson River Saranac Pellet Stove Manual, Alesis Midiverb 1 Manual, User Manual For Samsung Galaxy Ace Duos S6802, Di Style Manual, R150f Transmission Repair Manual, Eos5d Mark 2 Manual. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. SpectraMax ® Plus 384 optics. 2. 4. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Effect of hypoxia on natriuretic peptides and VEGF production in AC16 cells. Novak, Nathan J. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. SpectraMax Multi-Mode Microplate Readers. Stat Fax 4700 Microstrip Reader. 0% and ± 0. ). This assay measures the two major sugars in wine, glucose and fructose. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. The Spectra Catalina 340 Automated Connect 12Volt or 24Volt Catalina 340C produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize. 5 mL sample or standard solution was well mixed with 0. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Acceptor filter: 665 nm. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. For more sample throughput, the system canThe optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). 5. Versteegh, Yoran H. measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. 1 0 100 80 60 40 20 0 4. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. 30. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). Donor: 620 nm. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. addresses the needs of life science researchers who quantify very small amounts of DNA, RNA, oligos, and proteins. reader (Molecular Devi ces). Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of. The intensity of red color formed in the assay was measured at a wavelength of 490 nm with an ELISA reader (SpectraMax 340, Molecular Device Co. 304 signal level saturation. Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). S. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Samples containing 25 μg of protein were separated on 12. Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. 1: SpectraMax Plus 384 components. Molecular Devices SpectraMax M5e Microplate Reader. Molecular Device M2e. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. Crowder, Ziqian Zeng, Alissa N. Enzyme activities at lower concentrations were assayed by using a LS50B fluorometric. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. 30. PerkinElmer offers a variety of Microplates For Absorbance and Colorimetric Assays. 4) was followed according to Vanacker et al. The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. 10. Statistical AnalysisConsult Molecular Devices's entire SpectraMax i3x catalogue on MedicalExpo. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). The SPECTRAmax 340PC can measure. , Sunnyvale, CA). ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Call 1-800-745-2710. , Sunnyvale, CA) at 490 nm. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The concentration of Alizarin Red S staining in the samples was determined by comparing the absorbance values with those. Warranty. 2 software was used to measure and calculate of the bioactivity activity studies. 2. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . Call 1-800-745-2710. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. Example: [Drawer]. Molecular Device M5e. 25–0. 2. Up for your consideration is a Molecular Devices SpectraMax 340 Microplate Spectrophotometer Model# 340 Condition is for parts or repair. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. -1 activity was determined MMP using an MMP-1 immunoassay kit (dY901; R&d Systems, Inc. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. VersaMax reads 96-well and strip-well plates. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. . 4. 6. The. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 315 can't find zero order. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. more on but is otherwise untested and sold AS-IS. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. 8. Polystyrene plates (384-well) were precoated overnight at 4°C with 2 μg/ml of murine, human, or chimeric RANKL. Here, we show how the SpectraMax® i3x and SpectraMax® iD5 Multi-Mode Microplate Readers are used to perform robust, no-wash saturation binding assays using HTRF. The cell death was calculated as follows:Absorbance was monitored at 450 nm (SpectraMax 340). Molecular Devices VersaMax Absorbance Microplate Reader, 340-850 nm, Molecular Devices (CAT#: STEM-SPO-0005-YJL) Labsystems Fluoroskan II Fluorescent Microplate Reader, 320 – 700 nm. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). or microplate reader (e. To screen for antigen specific IgG, 50 μL/well of biotinylated antigen at a concentration of 20-50 ng/mL was added and incubated for one hour at RT. The SPECTRAmax 340PC can mea- TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes acrossawiderangeofconcentrations. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. 2. 1% N-1-naphtylethylenediamine dihydrochloride, 2. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The reaction was stopped with 1 M hydrochloric acid after 15 min. The GH Pro 340 Supplemental Greenhouse LED grow lighting is designed with greenhouse operations in mind. Cytotoxicity was determined by measuring the release of LDH. Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. 2. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. SpectraMax 340 from Molecular Devices). The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. The plate was covered and incubated for one hour at room temperature. 5. Fully tested with WarrantyThe SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. To measure the cell biomass (g/L),. 2 - 3. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. Create custom fluorescence assays without having to purchase expensive filter sets. TGFα release was calculated as described in the Results. The reaction mixture was incubated for 5 min. 20. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. D. Then the samples were incubated for 1 h with human IL6 detecting antibody and then with HRP conjugate for 40 min. Fios Router Setup Manual, Losi Xx4 We Manual, Statics Hibbeler Solutions Manual 12th Edition, Nokia Hs 23 Manual, Spectramax 340 Manual, Nolan N102 User Manual, Ship Security Officer Manual yunqian 4. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). We also offer depot Repair Service from our base of operations in Southern California (San Diego). Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. , Sunnyvale, CA). The activity of glutathione peroxidase (GPX) was quantified spectrophotometrically at 340 nm, following the method of Takeda et al. Each experiment was performed in duplicate and repeated at. 6 g sodium acetate trihydrate and 2. Although all samples initially were analyzed at the same volume, the volume of sample. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). LAL Reagent Grade ™ Multi-well Plates (Lonza cat. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. , Sunnyvale, CA). DNA extraction from blood meal ELISA homogenate. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. plates can be used in the SPECTRAmax PLUS 384. When necessary, samples were. Ehrlich assay for quantification of 5-ALA A 200 μL 5-ALA sample was mixed with 200 μL sodium acetate (pH 4. Oxidative stress induction by H 2 O. The extinction coefficient at 280 nm used for determining the concentration of Rsp3690 was calculated to be 28,795 M –1 cm –1. 190–850 nm <± 1. 5 stars - 1602 reviewsThis assay typically develops in 1. (1993). SPECTRAmax® 190. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. 340-850 nm: Wavelength Selection: Monochromator, turnable in 1 nm increments:. Data were expressed as the mean percent viable cell vs. Amino Acid Analysis. Anti-proteolytic activity. The VersaMax and SpectraMax 190 read 96-well microplates. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. A 0. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. After 72 h, 20 μl of 5 mg/ml MTT solution was added to each well and plates were incubated for 3 h at 37°C. SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. High fg levels of Streptavidin High fg levels of Streptavidin Exceeds Cisbio certification requirements TRF-based with 340/80 nm EX and 616/10 nm EM AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm. Endpoint, kinetic, spectrum. 313 reference gain check fail. g. Glutathione reductase (GR) activity was measured, according to Smith et al. Triton X-100 (see Note 2). The metals were preincubated with A for 5 min when added before heme. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Quick View. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Call 1-800-745-2710. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. Readings were made in a microplate spectrophotometer (SpectraMAX 340, Molecular Devices, Sunnyvale, California, USA) with wavelength set to 450 nm and correction set. Thin layer chromatography (TLC) was. TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive. This was followed by an additional incubation at 37 °C for 30–60 min. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. FACS analysis. TDP-43 stress granular cells were treated with 70% ice-cold. Each experiment was performed in duplicate and repeated at. The plates were read using an ELISA microplate reader (Molecular Devices SPECTRAMAX 340 PC 384, Sunnyvale, California, USA) at 450 nm. Not. Acceptor filter: 665/10 nm. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter $1,000. control or celatrol-treated cells. 2 Chemical Reagents 1. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. 0 1. SpectraMax QuickDrop Micro-Volume Spectrophotometer. 305 reference level saturation. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. One plate carrier adapter is provided with the instrument. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. ac. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Description. 94 g calcium chloride dihydrate (both. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. 5, 2 mM pNPP, and recombinant enzymes, PTP1B activities were continuously monitored on a SpectraMax 340 Microplate Reader (SpectraMax, Silicon Valley, CA, USA) at 405 nm for 2 min at 30°C. 0200-6117. Figure 1. The results are expressed as the percentage inhibition of BrdU incorporation compared to the untreated control. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). Molecular Devices SpectraMax M5e Microplate Reader. All assays were performed using a sample size of 12, and the absorbance at 425 nm was measured by using a SpectraMax 340 multiwell plate reader (Molecular Devices, Sunnyvale, CA). TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. VersaMax reads 96-well and strip-well plates. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. One plate carrier adapter is provided with the instrument. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. 3: The cuvette chamber. Examples: “Press carefully to engage,” “Do not press down. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Following cell culture under normoxic or hypoxic con-ditions, culture medium samples were obtained on completing the incubation period to measure atrial natriuretic peptide. Other technologies such as imaging. Absorbance was measured at 450 nm by using a SpectraMax 340 microplate spectrophotometer (Molecular Device). Molecular Devices Spectramax 340PC instructions. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. using SpectraMax i3 (Molecular devices, San Jose, USA). One plate carrier adapter is provided with the instrument. Supplier: Molecular Devices. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- pathways and protein activation and expression in one system. Homeostatic model assessment for insulin. After washing, the dye was eluted with 3% HCl, and the absorbance measured with a SpectraMax 340 microtiter plates reader at 665 nm. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. Molecular Devices SpectraMax Gemini XS Microplate Reader, 250-850nm, Molecular Devices, for detection of samples in microplates. 340 nm / 80 nm. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Products : SpectraMax Plus 384 reader,. 5% phosphoric acid) in microplates at room temperature for 10 min. The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. The concentration of TNFα in the experimental samples was calculated from a mouse TNFα standard curve of 15–2000 pg/ml. SpectraMax Multi-Mode Microplate Readers. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. Alves, Amelia K. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. \r plates can be used in the SPECTRAmax PLUS 384. The absorbance at 405 nm was determined using a microplate reader (spectraMAX 340). edu Contact Phone: 216-368-8962. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). If depot service is the best option for you we will assist you with logistics to help you keep.